Product Name:
Mouse Collagen Type Ⅱ (COLⅡ) ELISA Kit
Cat.#:
JL11584
Regulatory Status:
Alternative:
COL2
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
3.12-200pg/mL
Sensitivity:
1.08pg/mL
Specificity:
This kit recognizes Mouse COLⅡ in samples. No significant cross-reactivity or interference between Mouse COLⅡ and analogues was observed.
Sample Type:
serum, plasma, tissue homogenates and other biological fluids
Assay Procedure:

Research Area:
Metabolism
Product Datasheets
| COLⅡ
Type II collagen is the basis for hyaline cartilage, including the articular cartilages at joint surfaces. It is formed by homotrimers of collagen, type II, alpha 1 chains.It makes up 50% of all protein in cartilage and 85–90% of collagen of articular cartilage.Type II collagen is organised into fibrils. This fibrillar network of collagen allows the cartilage to entrap the proteoglycan aggregate, as well as providing tensile strength to the tissue. Oral administration of native type II collagen induces oral tolerance to pathological immune responses and may be useful in arthritis.| Typical data
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.Concentration (pg/mL) | 200 | 100 | 50 | 25 | 12.5 | 6.25 | 3.12 | 0 |
OD | 2.6 | 1.9 | 1.24 | 0.75 | 0.46 | 0.31 | 0.22 | 0.07 |
Corrected OD | 2.53 | 1.83 | 1.17 | 0.68 | 0.39 | 0.24 | 0.15 | - |
| Repeatability
Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%| Recovery
The recovery of Mouse COLⅡ spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.Sample Type | Range(%) | Average Recovery(%) |
Serum(n=8) | 84-101 | 96 |
Plasma(n=8) | 92-105 | 102 |
Cell Culture Supernates(n=8) | 96-108 | 105 |
| Linearity
Samples were spiked with high concentrations of Mouse COLⅡ and diluted with Universal Diluent to produce samples with values within the range of the assay.Serum(n=4) | Plasma(n=4) | Cell Culture Supernates(n=4) | ||
1 : 2 | Range (%) | 84-95 | 88-96 | 90-110 |
Average (%) | 91 | 93 | 96 | |
1 : 4 | Range (%) | 89-103 | 88-108 | 105-115 |
Average (%) | 94 | 98 | 108 |






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